UDC 631.522
For breeding achievements, the method of identifying varieties is of great importance, on the basis of which an opinion is given on their novelty, distinctiveness and legal protection. The study presents the application of phenotypic markers on the white clover culture. The search for genotypes having distinctive phenotypic features is carried out at all stages of the breeding process. There is a working collection of phenotypic markers on the pattern of the leaf plate, anthocyanic color of the leaves and multi-leaf. Iso-genic populations with a specific marker trait are created from selected individual plants or groups of plants through manual pollination and repeated selection. Isogenic populations with marker traits have been created: "filled white leaf plate mark", "elongated leaf plate mark", "absence of a white mark on the leaf plate", and "pink inflorescence color". Phenotypically marked samples can become varieties them-selves or be used for marking promising breeding material. The experiment demonstrates the possibility of phenotypically marking promising breeding material of white clover using the marker trait "pink inflo-rescence color". It has been shown that the inflorescence color trait is transmitted in the F1 and F2 genera-tions.
white clover, marking, phenotype, selection, breeding
1. Taylor N. L. Registration of gene marker germplasm for red clover (Reg. CP1 for CP) // Crop Science. – 1982. – Vol. 22. – № 6. – P. 1269.
2. Matveeva O. S. Izuchenie sortovogo raznoobraziya na otlichimost', odnorodnost' i stabil'-nost' priznakov i sozdanie geneticheski markirovannogo selekcionnogo materiala klevera lugovogo (Trifolium rratense L.) : dis. kand. s.-h. nauk. – Moskva, 2004. – 163 s. – EDN NMQTJP.
3. Inheritance and frequencies of leaf markings in white clover / H. L. Carnahan, H. D. Hill, A. A. Han-son, K. G. Brown // Journal of Heredity. – 1955. – Vol. 46. – № 3. – Pp. 109–114. – EDN IRQGKH.
4. MYB-mediated regulation of anthocyanin biosynthesis / H. Yan, X. Pei, H. Zhang, et al. // Interna-tional Journal of Molecular Sciences. – 2021. – Vol. 22. – № 6. – Pp. 3103. – EDN WOANSG. – DOIhttps://doi.org/10.3390/ijms22063103.
5. Brewbaker J. L. V-leaf markings of white clover // Journal of Heredity. – 1955. – Vol. 46. – № 3. – Pp. 115–123. – EDN HVBQEK.
6. Genetic nomenclature in clovers and special purpose legumes: I. Red and white clover / K. H. Quesenberry, R. R. Smith, N. L. Taylor, et al. // Crop Science. – 1991 – Vol. 31. – № 4. – Pp. 861–867. – DOIhttps://doi.org/10.2135/cropsci1991.0011183X003100040003x.
7. Leaf trait coloration in white clover and molecular mapping of the red midrib and leaflet number traits / R. M. Tashiro, Y. Han, M. J. Monteros, et al. // Crop Science. – 2010. – Vol. 50. – № 4. – Pp. 1260–1268. – DOIhttps://doi.org/10.2135/cropsci2009.08.0457.
8. Zohary M., Heller D. The genus Trifolium. – Jerusalem : Israel Academy of Sciences and Humanities, 1984. Pp. 606.
9. Anthocyanin leaf markings are regulated by a family of R2R3-MYB genes in the genus Trifolium / N. W. Albert, A. G. Griffiths, G. R. Cousins, et al. // New Phytologist. – 2015. – Vol. 205. – № 2. – Pp. 882–893. – DOIhttps://doi.org/10.1111/nph.13100.
10. Corkill L. Leaf markings in white clover // Journal of Heredity. – 1971. – Vol. 62. – № 5. – Pp. 307–310. – EDN IRSVHX.
11. Metodicheskie ukazaniya po selekcii i pervichnomu semenovodstvu klevera / Pod red. Z. Sh. Shamsutdinova, A. S. Novoselovoy, S. A. Bekuzarovoy. – M. : Tipografiya Rossel'hoza-kademii, 2002. – 72 s. – EDN RSNVNH.
12. Malyshev L. L., Dzyubenko E. A., Malysheva N. Yu. Metodicheskie ukazaniya po rabote s kol-lekciey kormovyh kul'tur. – SPb. : VIR, 2024. – 52 s. – EDN KGACPO. – DOIhttps://doi.org/10.30901/978-5-907780-10-1.
13. Muhina N. A., Stankevich A. K. Kul'turnaya flora, mnogoletnie bobovye travy (klever, lyad-venec). – M. : Kolos, 1993. – 336 s.



