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 <front>
  <journal-meta>
   <journal-id journal-id-type="publisher-id">Adaptive Fodder Production</journal-id>
   <journal-title-group>
    <journal-title xml:lang="en">Adaptive Fodder Production</journal-title>
    <trans-title-group xml:lang="ru">
     <trans-title>Адаптивное кормопроизводство</trans-title>
    </trans-title-group>
   </journal-title-group>
   <issn publication-format="online">2222-5366</issn>
  </journal-meta>
  <article-meta>
   <article-id pub-id-type="publisher-id">91454</article-id>
   <article-id pub-id-type="doi">10.33814/AFP-2222-5366-2024-3-24-29</article-id>
   <article-categories>
    <subj-group subj-group-type="toc-heading" xml:lang="ru">
     <subject>ЭКСПЕРИМЕНТАЛЬНО-ИССЛЕДОВАТЕЛЬСКИЕ РАБОТЫ</subject>
    </subj-group>
    <subj-group subj-group-type="toc-heading" xml:lang="en">
     <subject>EXPERIMENTAL RESEARCH WORK</subject>
    </subj-group>
    <subj-group>
     <subject>ЭКСПЕРИМЕНТАЛЬНО-ИССЛЕДОВАТЕЛЬСКИЕ РАБОТЫ</subject>
    </subj-group>
   </article-categories>
   <title-group>
    <article-title xml:lang="en">Study on inheritance pattern and nutritional analysis of ATGRF2 transgenic 'Burgaltai' (Medicago varia Marthz)</article-title>
    <trans-title-group xml:lang="ru">
     <trans-title>Наследование AtGRF2 гена и химический состав сухого вещества трансгенного сорта люцерны изменчивой (Medicago varia Marthz) Бургалтай</trans-title>
    </trans-title-group>
   </title-group>
   <contrib-group content-type="authors">
    <contrib contrib-type="author">
     <name-alternatives>
      <name xml:lang="ru">
       <surname>Ууганзая</surname>
       <given-names>М. </given-names>
      </name>
      <name xml:lang="en">
       <surname>Uuganzaya</surname>
       <given-names>M. </given-names>
      </name>
     </name-alternatives>
     <xref ref-type="aff" rid="aff-1"/>
    </contrib>
    <contrib contrib-type="author">
     <name-alternatives>
      <name xml:lang="ru">
       <surname>Альтанцеце</surname>
       <given-names>Л </given-names>
      </name>
      <name xml:lang="en">
       <surname>Altantsetse</surname>
       <given-names>L </given-names>
      </name>
     </name-alternatives>
     <xref ref-type="aff" rid="aff-2"/>
    </contrib>
    <contrib contrib-type="author">
     <name-alternatives>
      <name xml:lang="ru">
       <surname>Баярсайхан</surname>
       <given-names>Ш </given-names>
      </name>
      <name xml:lang="en">
       <surname>Bayarsaikhan</surname>
       <given-names>Sh </given-names>
      </name>
     </name-alternatives>
     <xref ref-type="aff" rid="aff-2"/>
    </contrib>
    <contrib contrib-type="author">
     <name-alternatives>
      <name xml:lang="ru">
       <surname>Энхчимег</surname>
       <given-names>В </given-names>
      </name>
      <name xml:lang="en">
       <surname>Enkhchimeg</surname>
       <given-names>V </given-names>
      </name>
     </name-alternatives>
     <xref ref-type="aff" rid="aff-3"/>
    </contrib>
   </contrib-group>
   <aff-alternatives id="aff-1">
    <aff>
     <institution xml:lang="ru">Research Institute of Animal Husbandry, Mongolian University of Life Sciences</institution>
     <country>Монголия</country>
    </aff>
    <aff>
     <institution xml:lang="en">Research Institute of Animal Husbandry, Mongolian University of Life Sciences</institution>
     <country>Mongolia</country>
    </aff>
   </aff-alternatives>
   <aff-alternatives id="aff-2">
    <aff>
     <institution xml:lang="ru">Research Institute of Animal Husbandry</institution>
     <city>Ulan Bator</city>
     <country>MN</country>
    </aff>
    <aff>
     <institution xml:lang="en">Research Institute of Animal Husbandry</institution>
     <city>Ulan Bator</city>
     <country>MN</country>
    </aff>
   </aff-alternatives>
   <aff-alternatives id="aff-3">
    <aff>
     <institution xml:lang="ru">Военный учебно-научный центр военно-воздушных сил «Военно-воздушная академия имени профессора Н. Е. Жуковского и Ю. А. Гагарина»</institution>
     <country>Россия</country>
    </aff>
    <aff>
     <institution xml:lang="en">Military Educational and Scientific Centre of the Air Force «Professor N.E. Zhukovsky and Y.A. Gagarin Air Force Academy»</institution>
     <country>Russian Federation</country>
    </aff>
   </aff-alternatives>
   <pub-date publication-format="print" date-type="pub" iso-8601-date="2024-12-04T09:34:20+03:00">
    <day>04</day>
    <month>12</month>
    <year>2024</year>
   </pub-date>
   <pub-date publication-format="electronic" date-type="pub" iso-8601-date="2024-12-04T09:34:20+03:00">
    <day>04</day>
    <month>12</month>
    <year>2024</year>
   </pub-date>
   <volume>2024</volume>
   <issue>3</issue>
   <fpage>24</fpage>
   <lpage>29</lpage>
   <history>
    <date date-type="received" iso-8601-date="2024-12-03T00:00:00+03:00">
     <day>03</day>
     <month>12</month>
     <year>2024</year>
    </date>
   </history>
   <self-uri xlink:href="https://adaptagro.editorum.ru/en/nauka/article/91454/view">https://adaptagro.editorum.ru/en/nauka/article/91454/view</self-uri>
   <abstract xml:lang="ru">
    <p>Сорт люцерны изменчивой Бургалтай является ценной кормовой культурой по поедаемости и питательности. Тем не менее, отечественные ученые считают, что у возделываемого в Монголии вида Medicago sp. недостатком является низкая облиственность (до 45%), которая в процессе развития растений люцерны резко снижается. Установлено, что высокая экспрессия AtGRF2 гена приводит к увеличению размеров листьев и семядолей по сравнению с диким типом. В исследовании показано, что у трансгенного сорта Бургалтай с геном AtGRF2 площадь листьев увеличилась на 0,52 см2, а процентное содержание листьев на растении — до 48,6%. Трансгенные линии показали соотношение расщепления 3:1 в потомстве T1 (χ2 = 0,04–0,42, p ≤ 0,52–0,84). Концентрация антибиотика гигромицина в селективных средах составляет 50 мг/л. Согласно результатам оценки корма, в трансгенном Бургалтае увеличилось содержание некоторых химических веществ, за исключением общего жира.</p>
   </abstract>
   <trans-abstract xml:lang="en">
    <p>Alfalfa (Medicago varia Marthz) 'Burgaltai' cultivar is one of the most important forages of its palatable and nutritional values. But still, national scientists had reported that there is lack of characteristic have been appeared in Mongolian cultivated Medicago sp., and it is the percentage of leaf in the yield is low (up to 45%) and it had decreased dramatically in development cycle. Over-expression of AtGRF2 resulted in larger leaves and cotyledons comparing to wild type. In this study, transgenic 'Burgaltai' with AtGRF2 gene had increased leaf area by 0.52 cm2 and leaves in percentage per bush up to 48.6%. The transgenic lines showed 3:1 segregation ratio in T1 progenies (χ2 = 0.04–0.42, p ≤ 0.52–0.84). Hygromycin antibiotic concentration is 50 mg/l for selective media. According to the feed evaluation, some chemical composition increased in transgenic 'Burgaltai' except total fat.</p>
   </trans-abstract>
   <kwd-group xml:lang="ru">
    <kwd>люцерна</kwd>
    <kwd>AtGRF2 ген</kwd>
    <kwd>трансгенный и дикий сорт Бургалтай</kwd>
    <kwd>антибиотик гигромицин</kwd>
   </kwd-group>
   <kwd-group xml:lang="en">
    <kwd>alfalfa</kwd>
    <kwd>AtGRF2 gene</kwd>
    <kwd>transgenic 'Burgaltai' and wild 'Burgaltai' varieties</kwd>
    <kwd>hygromycin antibiotic</kwd>
   </kwd-group>
  </article-meta>
 </front>
 <body>
  <p></p>
 </body>
 <back>
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