05550nam#a2200397#i#4500001000500000005001700005008004000022020002300062044000900085080012300094100015200217100010500369100029400474100015800768100015200926245013101078260009101209300001101300500122901311510013602540510018902676510012002865510027402985510023703259510014503496510012403641510018703765510017903952510018404131510024404315510016504559510016204724510020604886533003305092856002705125202120241018135856.5  20201112d2020####ek#y0engy0150####ca##$a978-5-6043194-9-9##$axxu##$aСельское хозяйство. Лесное хозяйство. Охота. Рыбное хозяйство. 63#1$aKlimenko, Irina Alexandrovna$aFederal State Budget Sciences Institution «Federal Williams Research Center of Forage Production and Agroecology»#1$aKozlov, Nikolay Nikolaevich$aFederal Williams Research Center of Forage Production and Agroecology#1$aKostenko, Sergey Ivanovich$aФедеральное государственное бюджетное научное учреждение «Федеральный научный центр кормопроизводства и агроэкологии имени В.Р. Вильямса»#1$aShamustakimova, Anastasia Olegovna$aFederal State Budget Sciences Institution «Federal Williams Research Center of Forage Production and Agroecology»#1$aMavlyutov, Yulian Muratovich$aFederal State Budget Sciences Institution «Federal Williams Research Center of Forage Production and Agroecology»00$aIdentification and certification of forage grasses (meadow clover, alfalfa, sowing and hop) based on DNA markers$cMonography1#$aLobnya$bFederal Williams Research Center of Forage Production and Agroecology$c2020##$a36 p.##$aA technology has been developed for DNA identification and certification of varieties of meadow clover (Trifolium pratense L.), alfalfa (Medicago varia Mart.), Sowing (M. sativa L.) and hop (M. lupuli-na L.) based on molecular analysis with using SSR and SRAP markers. The recommendations contain a description of the sequence of experiments and protocols for DNA typing procedures. The presented methods were developed by the authors on the basis of their own experimental research and using the data available in the literature. A characteristic of informative primers for each marking system is given, a set of DNA identification markers is proposed, and unique molecular genetic formulas of varieties are drawn up as the basis for a reference genetic passport. Methodological recommendations were prepared with the aim of mastering the technology of DNA certification of forage grasses in practice. Designed for managers and specialists of research and control laboratories, can serve as a textbook for students and postgraduates in specialized specialties.$aforage crops, red clover, alfalfa, genetic diversity, DNA-fingerprinting, microsatellite analysis, molecular-genetic certification$a10.33814/978-5-6043194-9-90#$aStrategiya nauchno-tehnologicheskogo razvitiya Rossiyskoy Federacii ot 01. 12. 2016 g. № 642. http://www.rcit.su/techinfoM1.html0#$aFederal'naya nauchno-tehnicheskaya programma razvitiya geneticheskih tehnologiy na 2019–2027 gody. Postanovlenie ot 22. 04. 2019 g. № 479. http://static.government.ru/media/files/0#$aGlik B., Pasternak Dzh. Molekulyarnaya biotehnologiya. Principy i primenenie / Per. s angl. M. : Mir, 2002. 589 s.0#$aKozlov N. N., Klimenko I. A. Tehnologicheskiy pasport kollekcii (regional'naya geneticheskaya kollekciya kormovyh rasteniy). SOP № 5 – «Sozdanie identifikacionnyh DNK-pasportov selekcionnyh dostizheniy kormovyh rasteniy» https://www.vniikormov.ru/pdf/tpk2018.pdf0#$aKutlunina N. A. Molekulyarno-geneticheskie metody v issledovanii rasteniy : ucheb.-metod. posobie; M-vo obrazovaniya i nauki Ros. Federacii, Ural. feder. un-t. Ekaterinburg : Izd-vo Ural. un-ta, 2017. 142 s. ISBN 978-5-7996-2142-1.0#$aOsnovy polimeraznoy cepnoy reakcii (PCR). Metodicheskoe posobie / sostavitel' V. V. Zorina. Moskva : OOO «DNK-Tehnologiya», 2012 g. 76 s.0#$aPadutov V. E., Baranov O. V., Voropaev E. V. Metody molekulyarno-geneticheskogo analiza. Minsk : Yunipol, 2007. 176 s.0#$aChesnokov Yu. V., Kocherina N. V., Kosolapov V. M. Molekulyarnye markery v populyacionnoy genetike i selekcii kul'turnyh rasteniy. M. : OOO «Ugreshskaya Tipografiya», 2019. 200 s.0#$aDon R. H., Cox P. T., Wainwright B. J., Baker K. and Mattick J. S. Touchdown PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res. 1991. 19: 4008.0#$aFu Yong-Bi. Applications of bulking in molecular characterization for plant germplasm: a critical review. Plant Genetic Resources. 2003. 1 (2-3), 161–167. DOI: 10.1079/PGR200320#$aLi G. and Quiros C.F. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theoretical and Applied Genetics. 2001. V. 103, p. 455-461.0#$aNei M. and Li W. H. 1979. Mathematical Model for Studying Genetic Variation in Terms of Restriction Endonucleases. Proc. Natal Acad. Sci. USA, 76: 5269–5273.0#$aSanger F., Coulson A. R. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase. J. Mol. Biol. 1975. Vol. 94, p. 444–448.0#$aSato S., Isobe S., Asamizu E., Ohmido N., et al. Comprehensive structural analysis of the genome of red clover (Trifolium pratense L.). DNA Research. 2005. 12(5): 301–364. DOI: 10.1093/dnares/dsi018##$aThere is an electronic copy4#$aadaptagro.editorum.ru