Adaptive Fodder Production Adaptive Fodder Production Адаптивное кормопроизводство 2222-5366 105369 10.33814/AFP-2222-5366-2025-3-27-34 ЭКСПЕРИМЕНТАЛЬНО-ИССЛЕДОВАТЕЛЬСКИЕ РАБОТЫ EXPERIMENTAL RESEARCH WORK ЭКСПЕРИМЕНТАЛЬНО-ИССЛЕДОВАТЕЛЬСКИЕ РАБОТЫ Effects of growth regulators and composition of nutrient media on in vitro regeneration of Juniperus sabina L. Влияние регуляторов роста и состава питательных сред на регенерацию in vitro Juniperus sabina L. Ууганзая М. Uuganzaya M. Альтантсекссет Лхамакин Altantsetseg Lkhamaachin Research Institute of Animal Husbandry, Mongolian University of Life Sciences Монголия Research Institute of Animal Husbandry, Mongolian University of Life Sciences Mongolia Forage Plant Tissue Culture Laboratory, Research Institute of Animal Husbandry, Mongolia Россия Forage Plant Tissue Culture Laboratory, Research Institute of Animal Husbandry, Mongolia Russian Federation 17 10 2025 17 10 2025 2025 3 27 34 14 10 2025 https://adaptagro.editorum.ru/en/nauka/article/105369/view

Приведены результаты разработки метода регенерации экономически важного для Монголии растения — можжевельника казацкого (Juniperius sabina L.). Регенерация растений проводилась с использованием эксплантов листьев/побегов длиной 1,5–2,0 см, собранных летом. Реакция побегов на эксплантах зависела от типа питательной среды и концентрации регулятора роста 6-бензиламинопурина (БАП). Высокоэффективное побегообразование получено при выращивании на среде Мурасиге и Скуга (MS), среде Мурасиге и Скуга с витамином В5 Гамборга (MSB5) и базальной среде Шенка и Хильдебрандта (SH) с добавлением 0–1 мг/л 6-бензиламинопурина (БАП) длиной 0,5–2,6 см. Наибольшая скорость образования корней составила 75% на среде SH с добавлением 0,5 мг/л нафтилуксусной кислоты (НУК). Наибольшая длина корней наблюдалась на базальной среде Шенка и Хильдебрандта (SH) без регулятора роста.

In this study we have to establish and optimize a regeneration for economically important Juniperius sabina L. in Mongolia. Plant regeneration were achieved with leaves/shoots explants collected in the summer with the length of 1.5–2.0 cm. Shoot responses on explants depended on nutrient medium types and the concentration of 6-benzilaminopurine (BAP) growth regulator. Highly efficient shoot formation was obtained when either on Murashige and Skoog (MS), Murashige and Skoog medium with Gamborg’s B5 vitamin (MSB5) and Schenk and Hildebrandt Basal (SH) medium supplemented with 0–1 mg/l 6-benzilaminopurine (BAP) with the length of 0.5–2.6 cm. The highest root induction rate was 75% on SH medium supplemented with 0.5 mg/l naphthalene acetic acid (NAA). The longest root length observed Schenk and Hildebrandt Basal (SH) medium without growth regulator.

 Juniperus sabina in vitro регенерация регулятор роста Juniperus sabina in vitro regeneration growth regulator

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